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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become observed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential function in advertising cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two supplemental splice variants have been reported but specific nucleotide sequence details is not still accessible.
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Blocking peptides are peptides that bind especially to your target antibody and block antibody binding. These peptide typically incorporates the epitope recognized with the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make certain the very best effects are...
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This information outlines further attainable optimization of mobile and immunoassay parameters to make certain the best possible results are...
Antibodies bound to the blocking peptide no longer bind for the epitope to the focus on protein. By evaluating the staining within the blocked antibody compared to the antibody by itself, one can see which staining is precise.
Concentrate on Facts Cyclin E belongs for the hugely conserved cyclin household, whose associates are characterised by a remarkable periodicity in protein abundance throughout the mobile cycle. Cyclins perform as regulators of CDK kinases. Diverse cyclins show distinct expression and degradation styles which lead towards the here temporal coordination of each and every mitotic function. Cyclin E forms a posh with and capabilities like a regulatory subunit of CDK2, whose action is required for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary and is degraded as cells progress through S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each antibodies...
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